Human Anti-CD34 Recombinant Antibody (TAB-150LC)
CAT#: TAB-150LC
This product is a recombinant human antibody that recognizes CD34 (cluster of differentiation 34). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Tested Data
Gene Expression
WB
Figure 1 Anti-Human CD34 Recombinant Antibody (TAB-150LC) in WB
Western blot analysis was performed with 0.1 µg (lane 1), 0.3 µg(lane 2) recombinant human CD34 onto a 12% Tris-HCl polyacrylamide gel. Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour.
DB
Figure 2 Anti-Human CD34 Recombinant Antibody (TAB-150LC) in Dot Blot
Dot Blot analysis of TAB-150LC was performed with human CD34 antigen.
TAB-150LC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 3 Anti-Human CD34 Recombinant Antibody (TAB-150LC) in ELISA
ELISA analysis of TAB-150LC was performed by coating with recombinant human CD34 antigen. The HRP-conjugated goat anti-human IgG was used as a secondary antibody.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36723/1751_G1_7_cr57fd220c70be0_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_8_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HUVEC TERT2, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174059-CD34
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Human IgG
- Specificity
- Human CD34 (cluster of differentiation 34)
- Species Reactivity
- Human
- Applications
- ELISA
- Related Disease
- Chronic myocardial ischemia
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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(Creative Biolabs Cat# TAB-150LC, RRID: AB_3111837)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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